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Monsters
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All monsters regenerate their hit points at varying rates. Bosses do so faster than regular monsters, and the rate increases with the difficulty level. Poison damage (easily added to physical damage from charms) and "Prevent Monster Heal" items will stop hit point regeneration entirely. Another option for stopping regeneration is the "open wounds" modifier on certain weapons and armor. Additionally, weapons can have "poison" or "open wound" modifiers placed on them through sockets.
===Difficult Difficulty Level Changes===
Monsters grow much more difficult on the Nightmare and Hell difficulty levels.